EMBRYOLOGY :: 藤井節郎記念医科学センター

　Laboratory of Embryology was established in December 2013 at Fujii Memorial Institute of Medical Sciences, Tokushima University, Japan. We are interested in understanding how many types of cells are generated during gastrulation.

Research interest
Lab. Members
Publications
Photos and movies
Japanese

News

- ■ Takemoto will present his recent research at the Mouse Molecular Genetics 2015 The conference will be held in Wellcome Trunst Conference Centre (Cambridge) from the 16th to the 19th of September. Mouse Molecular Genetics 2015 http://conf.hinxton.wellcome.ac.uk/advancedcourses/MMG2015FINALPROG.pdf

■ A new paper was published in Scientific Reports. We established a method to generate mutant mice using the CRIPSR/Cas9 system and electroporation. This work was published on the 11th of June in Scientific Reports.http://www.nature.com/srep/2015/150611/srep11315/full/srep11315.html

Research interest

　Embryos develop by generating many types of cell. One of the most dynamic events that generate a variety of cell type during embryogenesis is gastrulation.Our group is interested in how many types of cell are generated during gastrulation.

So far, we have investigated how neural plate cells are generated during gastrulation, by analyzing the regulatory mechanism of early neural marker gene, Sox2. We have clarified that 1.Neural plate cells and paraxial mesoderm cells are generated from their common precursor, axial stem cells. 2.The fate of axial stem cells, neural plate cells or mesodermal cells, are determined by transcription factors, Sox2 and Tbx6, respectively. 3.The three germ layers describe spatial organization of tissues, but do not indicate the process of tissue derivation. Currently, we are studying how the axial stem cells are generated and maintained in embryos, and how the anterior-posterior axis elongates by generating neural plate cell and mesodermal cells.

Lab. Members　※I assume ,@ mark (@) for spam mail measures.

Tatsuya Takemoto, Ph. D. Professor (PI): TEL: +81-88-634-6412 E-mail: takemoto.tatsuya(@)tokushima-u.ac.jp

Shinichi Hayashi, Ph. D. Assistant Professor: TEL: +81-88-634-6463 E-mail: hayashi.shinichi(@)tokushima-u.ac.jp

suzuki

Technical Staff Hitomi Suzuki: TEL: +81-88-634-6406; E-mail: hsuzuki(@)tokushima-u.ac.jp

saito

Technical Staff Erika Saito: TEL: +81-88-634-6404; E-mail: saitou.erika(@)tokushima-u.ac.jp

shimizu

Secretary Yuko Shimizu: TEL: +81-88-634-6407; E-mail:shimizu.yuko(@)tokushima-u.ac.jp

Publications

TATSUYA Takemoto

（Since 2004,* = corresponding author）
■	Uddin MM, Ohigashi I, Motosugi R, Nakayama T, Sakata M, Hamazaki J, Nishito Y, Rode I, Tanaka K, Takemoto T, Murata S, Takahama Y. Foxn1-β5t transcriptional axis controls CD8+ T-cell production in the thymus. Nat Commun. 8:14419 (2017).
■	Tanihara F, Takemoto T, Kitagawa E, Rao S, Do L, Onishi A, Yamashita Y, Kosugi C, Suzuki H, Sembon S, Suzuki S, Nakai M, Hashimoto M, Yasue A, Matsuhisa M, Noji N, Fujimura T, Fuchimoto Di, Otoi T. Somatic cell reprogramming-free generation of genetically modified pigs. Science Advances. 2 (9) e1600803 (2016).
■	Do L, Wittayarat M, Terazono T, Sato Y, Taniguchi M, Tanihara F, Takemoto T, Kazuki Y, Kazuki K, Oshimura M, Otoi T*. Effects of duration of electric pulse on in vitro development of cloned cat embryos with human artificial chromosome vector. Reprod Domest Anim. (2016) In press.
■	Hashimoto M, Yamashita Y, Takemoto T*.Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. Dev. Biol. 418: 1-9 (2016).
■	Kondoh H, Takada S, Takemoto T. Axial level-dependent molecular and cellular mechanisms underlying the genesis of the embryonic neural plate. Dev Growth Differ.58(5): 427-436 (2016).
■	Takemoto T, Abe T, Kiyonari H, Nakao K, Furuta Y, Suzuki H, Takada S, Fujimori T, Kondoh H. R26-WntVis reporter mice showing graded response to Wnt signal levels. Genes Cells. 21(6): 661-669 (2016).
■	Takemoto T, Abe T, Kiyonari H, Nakao K, Furuta Y, Suzuki H, Takada S, Fujimori T, Kondoh H. R26-WntVis reporter mice showing graded response to Wnt signal levels. Genes Cells. DOI: 10.1111/gtc.12364 (2016).
■	Morita Y, Taniguchi M, Tanihara F, Ito A, Namula Z, DO LT, Takagi M, Takemoto T, Otoi T*. The optimal period of Ca-EDTA treatment for parthenogenetic activation of porcine oocytes during maturation culture. J Vet Med Sci. (2016).
■	Hashimoto, M. and Takemoto, T*. Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Sci. Rep. 5, 11315; doi: 10.1038/srep11315 (2015).
■	Takemoto T*. Regulation of axial stem cells deriving neural and mesodermal tissues during posterior axial elongation. In “New Principles in Developmental Processes” (eds. Kondoh H. and Kuroiwa A.), Springer, pp. 85-96 (2014).
■	Yoshida M, Uchikawa M, Rizzoti K, Lovel-Badge R, Takemoto T,Kondoh H*. Regulation of mesodermal precursor production by low-level expression of B1 Sox genes in the caudal lateral epiblast. Mech Dev. 132: 59-68 (2014)
■	Takemoto T*. The mechanism of cell fate choice between neural and mesodermal development during early embryogenesis. Congenit Anom. 53: 61?66 (2013).
■	Nishimura N, Kamimura Y, Ishida Y, Takemoto T, Kondoh H, Uchikawa M*. A systematic survey and characterization of enhancers that regulate Sox3 in neuro-sensory development in comparison with Sox2 enhancers. Biology. 1(3): 714-735 (2012).
■	Kondoh H*, Takemoto T. Axial stem cells deriving both posterior neural and mesodermal tissues during gastrulation. Curr Opin Genet Dev. 22: 1-7 (2012).
■	Takemoto T, Uchikawa M, Yoshida M, Bell DM, Lovell-Badge R, Papaioannou VE, Kondoh H*. Tbx6-dependent Sox2 regulation determines neural or mesodermal fate in axial stem cells. Nature. 470(7334): 394-398 (2011).
■	Iwafuchi-Doi M, Yoshida Y, Onichtchouk D, Leichsenring M, Driever W, Takemoto T, Uchikawa M, Kamachi Y, Kondoh H*. The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates. Dev Biol. 352(2): 354-366 (2011).
■	Uchikawa M, Yoshida M, Iwafuchi-Doi M, Matsuda K, Ishida Y, Takemoto T, Kondoh H*. B1 and B2 Sox gene expression during neural plate development in chicken and mouse embryos: Universal versus species-dependent features. Dev Growth Differ. 53(6): 761-771 (2011).
■	Kamachi Y, Iwafuchi M, Okuda Y, Takemoto T, Uchikawa M, Kondoh H*. Evolution of non-coding regulatory sequences involved in the developmental process: reflection of differential employment of paralogous genes as highlighted by Sox2 and group B1 Sox genes. Proc Jpn Acad Ser B Phys Biol Sci. 85(2):55-68 (2009).
■	Uchikawa M*, Takemoto T. Enhancer analysis: Strategies for locus-wide identification and systematic analysis of enhancers using chicken embryo electroporation. In “Electroporation and Sonoporation in Developmental Biology” (ed. Nakamura H.), Springer, pp. 55-72 (2009).
■	Takemoto T, Uchikawa M, Kamachi Y,Kondoh H*. Convergence of Wnt and FGF signals in the genesis of posterior neural plate through activation of the Sox2 enhancer N-1. Development. 133(2): 297-306 (2006).
■	Uchikawa M, Takemoto T, Kamachi Y, Kondoh H*. Efficient identification of regulatory sequences in the chicken genome by a powerful combination of embryo electroporation and genome comparison. Mech Dev. 121(9): 1145-1158 (2004).
■	Uchikawa M, Ishida Y, Takemoto T, Kamachi Y, Kondoh H*. Functional analysis of chicken Sox2 enhancers highlights an array of diverse regulatory elements that are conserved in mammals. Dev Cell. 4: 509-519 (2003).

SHINICHI Hayashi

■	Hayashi S, Nakahata Y, Kohno K, Matsui T, Bessho Y. Presomitic mesoderm-specific expression of the transcriptional repressor Hes7 is controlled by E-box, T-box, and Notch signaling pathway Journal of Biological Chemistry. in press
■	Hasugata R, Hayashi S, Kawasumi-Kita A, Sakamoto J, Kamei Y, Yokoyama H. Infrared Laser-Mediated Gene Induction at the Single-Cell Level in the Regenerating Tail of Xenopus laevis Tadpoles. in press
■	Seki R, C Li, Q Fang, Hayashi S, Egawa S, J Hu, L Xu, H Pan, Kondo M, Sato T, Matsubara H, Kamiyama N, Kitajima K, Saito D, Y Liu, M. Thomas P. Gilbert, Qi Zhou, X Xu, Shiroishi T, Irie N, Tamura K & G Zhang.Functional roles of Aves class-specific cis-regulatory elements on macroevolution of bird-specific features. Nature Communications. 8. 14229. (2017).
■	Yu X, Kawakami H, Tahara N, Olmer M, Hayashi S, Akiyama R, Bagchi A, Lotz M, Kawakami Y. Expression of Noggin and Gremlin1 and its implications in fine-tuning BMP activities in mouse cartilage tissues. J. Orth. Res. Nov. 1 (2016).
■	Hayashi S, Akiyama R, Wong J, Tahara N, Kawakami H, Kawakami Y. Gata6-Dependent GLI3 Repressor Function is Essential in Anterior Limb Progenitor Cells for Proper Limb Development. PLoS Genet. 12. e1006138. (2016).
■	Kawasumi-Kita A, Hayashi T, Kobayashi T, Nagayama T, Hayashi S, Kamei Y, Morishita Y, Takeuchi T, Tamura K, Yokoyama H. Application of local gene induction by infrared laser‐mediated microscope and temperature stimulator to amphibian regeneration study. Dev. Growth Differ. 57. 601-613. (2015).
■	Hayashi S, Kawaguchi A, Uchiyama I, Kawasumi-Kita A, Kobayashi T, Nishide H, Tsutsumi R, Tsuru K, Inoue T, Ogino H, Agata K, Tamura K, Yokoyama H. Epigenetic Modification Maintains Intrinsic Limb-Cell Identity in Xenopus Limb Bud Regeneration. Dev. Biol. 406. 271-282. (2015).
■	Hayashi S, Kobayashi T, Yano T, Kamiyama N, Egawa S, Seki R, Takizawa K, Okabe M, Yokoyama H, Tamura K. Evidence for an amphibian sixth digit. Zool. Let. 1. 17. (2015).
■	Hayashi S, Ochi H, Ogino H, Kawasumi A, Kamei Y, Tamura K, Yokoyama H. Transcriptional regulators in the Hippo signaling pathway control organ growth in Xenopus tadpole tail regeneration. Dev. Biol. 396. 1. 31-41. (2014).
■	Hayashi S, Tamura K, Yokoyama H. Yap1, transcription regulator in the Hippo signaling pathway, is required for Xenopus limb bud regeneration. Dev. Biol. 388, 57-67. (2014).
■	Kawasumi A, Sagawa N, Hayashi S, Yokoyama H, Tamura K. Wound healing in mammals and amphibians: Toward limb regeneration in mammals. Current Topics in Microbiology and Immunology. 367. 33-49. (2013).
■	Hayashi S, Shimoda T, Nakajima M, Tsukada Y, Sakumura Y, Dale JK, Maroto M, Kohno K, Matsui T, Bessho Y. Sprouty4, an FGF Inhibitor, Displays Cyclic Gene Expression under the Control of the Notch Segmentation Clock in the Mouse PSM. PLoS ONE. 4, e5603, (2009). contributed equally
■	Ferjentsik Z, Hayashi S, Dale JK, Bessho Y, Herreman A, De Strooper B, del Monte G, de la Pompa JL, Maroto M. Notch Is a Critical Component of the Mouse Somitogenesis Oscillator and Is Essential for the Formation of the Somites. PLoS Genet. 5, e1000662, (2009).
■	Hayashi S, Inoue A. Cardiomyocytes Re-enter the Cell Cycle and Contribute to Heart Development after Differentiation from Cardiac Progenitors Dev. Growth. Differ. 49, 229-239, (2007).